SRA II - Histology

Histology technician who supports research projects by performing histological procedures, including tissue preparation, sectioning, standard staining, immunostaining, imaging, and image quantification. Design experiments, characterize tissue and antibodies, image analysis, prepare and give presentations.

Carry out immunohistochemical procedures:

Preparation of tissue including grossing and embedding

Sectioning tissue using various equipment including cryostat, microtome, vibratome, and sliding-frozen microtome

High volume automated and manual Immunostaining

Experience with automatic slide stainers such as Leica Bond Rx and Biocare IntelliPATH FLX a plus

Experience with chromogenic and fluorescence immunodetection (single and multiplex)

Manual and automatic cover-slipping

Capturing images with fluorescence and brightfield microscopy

Experience with high throughput slide scanners such as Hamamatsu Nanozoomer and Zeiss Axioscan a plus

Carry out histological staining such as H&E, nuclear counterstaining, and other standard histological stains

Design and troubleshoot experiments, analyze data, and present in meetings with guidance from senior staff

Use of a microscope to examine the quality of staining, perform standard microscopic tasks, view samples and produce high quality images

Other laboratory tasks include:

Shelving and inventory of biospecimens and reagents

Maintaining reagent stocks and ordering supplies

Keeping laboratory in order and making sure equipment is well maintained

Work with CRO for outsourcing of tissue embedding and sectioning, when necessary

Collaborate and coordinate sample sharing with other research groups

Education and/or Experience:

MS in Biology or similar discipline with at least 4 years of industry experience or BA/BS with 6 years of relevant experience demonstrating direct participation of drug discovery efforts by IHC and other tissue-based techniques; ASCP certification (HT or HTL) certification is desirable

Experience in performing, optimizing, and implementing qualitative and quantitative tissue-based methodologies ranging from tissue handling and processing, sectioning with microtome or cryostat, histochemical staining is required

Experience with standard and advanced microscopy and digital pathology is required

Proficient at employing whole-slide automated image analysis (e.g., HALO and Definiens) and analyzing large data sets using statistical and data visualization programs (e.g., Prism, Spotfire) to quantify tissue-based efficacy markers is preferred

Understanding of mouse and human comparative microscopic anatomy, characterization of transgenic animals

Familiar with analysis and interpretation of data generated from tissue-based target expression studies and with statistical approaches and successful presentation of pathology projects at cross-functional team meetings is highly desirable